Part:BBa_K5057012
His-tagged Sushi S1 with extracellular localization sequence HSTII
Sushi S1 is an antimicrobial cationic peptide composed of 34 amino acids, derived from the lipopolysaccharide (LPS)-binding region of Factor C found in horseshoe crabs. It targets bacterial membranes through four successive steps in the bactericidal process: 1) Binding, primarily mediated by charged residues in the peptide; 2) Peptide association; 3) Membrane disruption, during which lipopolysaccharides remain intact; and 4) Lysis, resulting from the leakage of cytosolic contents through large membrane defects.
In our experiments, we investigated the effect of intracellular expression of HSTII-Sushi S1 (Biobricks: BBa_K5057002 - HSTII as a basic part, BBa_K5057004 - Sushi S1 as basic part, BBa_K5057007 HSTII-Sushi S1 as composite part) encoded on pET22b(+) backbone on the viability of bacterial cultures. For verification purposes, we created a His-tagged version of Sushi S1 that can be purified via affinity chromatography and detected on a western blot.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Yau YH, Ho B, Tan NS, Ng ML, Ding JL. High therapeutic index of factor C Sushi peptides: potent antimicrobials against Pseudomonas aeruginosa. Antimicrob Agents Chemother [Internet]. 2001;45(10):2820–5. Available from: http://dx.doi.org/10.1128/AAC.45.10.2820-2825.2001
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